Plotter Microarray Imager

This straightforward imaging technique is fast, quantitative, accurate and scaleable for high throughput. It is also portable and can be configured on standard microarray fabrication instrumentation and gantries.

Its also and can be configured with epi-fluorescence and filter wheels.

This demonstrates the utility of configuring a microarray spotter for high sensitive quantitative fluorescent imaging of high density microarrays. For this example, one slide was spotted with different concentrations of Cy3 labeled antibody. The same slide was compared using a Packard confocal laser microarray scanner.

Sorry for the long loading time, there's alot of data that demonstrates how well this works.

Video uScope

PMT Laser Scanner

Comparative Data - % Signal Saturation Titration Study

Dye Conc	Video uScope 100mW 532nm	PMT Scanner %80L %55PMT	PMT Scanner %80L %65PMT	PMT Scanner %80L %75PMT
3.3uM	Sat: % 91.338 CV: % 1.113	Sat: % 96.480 CV: % 2.519	Sat: % 97.083 CV: % 0.875	Sat: % 96.410 CV: % 0.907
820nM	Sat: % 91.999 CV: % 2.712	Sat: % 78.905 CV: % 12.237	Sat: % 92.263 CV: % 5.910	Sat: % 97.274 CV: % 1.063
200nM	Sat: % 70.504 CV: % 10.892	Sat: % 25.021 CV: % 24.004	Sat: % 69.649 CV: % 17.767	Sat: % 85.148 CV: % 4.104
50nM	Sat: % 33.240 CV: % 19.797	Sat: % 5.517 CV: % 24.906	Sat: % 16.839 CV: % 25.133	Sat: % 43.450 CV: % 24.606
13nM	Sat: % 18.136 CV: % 19.958	Sat: % 1.639 CV: % 28.856	Sat: % 5.426 CV: % 27.995	Sat: % 13.986 CV: % 28.827

Nano-Plotter Imaging Raw Data

Annotation	Signal	%CV Signal	Background	%CV Background	Sig:Noise	Spot Images
3.3uM	232.913	1.113	92.969	16.801	2.568
820nM	234.598	2.712	43.835	22.430	5.705
200nM	179.786	10.892	30.052	21.320	6.181
50nM	84.761	19.797	21.462	31.325	4.217
13nM	46.246	19.958	18.599	29.432	2.654
3.2nM	35.920	29.280	15.062	30.481	2.499
800pM	36.399	59.720	14.063	30.211	2.665
200pM	33.102	28.530	14.129	31.864	2.464

Packard Scanner Data

80 Laser Power 55 PMT Data

Annotation	Signal	%CV Signal	Background	%CV Background	Sig:Noise	Spot Images
3.3uM	63054.338	2.519	2042.379	19.819	31.836
820nM	51568.060	12.237	3134.935	13.635	16.507
200nM	16352.281	24.004	1254.781	27.260	13.234
50nM	3605.520	24.906	564.459	29.919	6.563
13nM	1071.209	28.856	416.173	40.461	2.763
3.2nM	632.410	33.162	361.908	37.265	1.893
800pM	596.968	70.515	339.798	37.038	1.809
200pM	585.078	56.143	345.954	35.218	1.757

80 Laser Power 65 PMT Data

Annotation	Signal	%CV Signal	Background	%CV Background	Sig:Noise	Spot Images
3.3uM	63448.396	0.875	4474.030	26.480	15.063
820nM	60298.704	5.910	3153.541	16.578	19.501
200nM	45519.291	17.767	3680.427	21.370	12.506
50nM	11005.167	25.133	1941.000	27.882	5.757
13nM	3545.979	27.995	1497.999	35.830	2.460
3.2nM	2153.847	32.649	1303.176	33.580	1.738
800pM	2106.375	82.320	1247.154	32.216	1.701
200pM	2024.328	51.551	1279.613	30.633	1.614

80 Laser Power 75 PMT Data

Annotation	Signal	%CV Signal	Background	%CV Background	Sig:Noise	Spot Images
3.3uM	63008.919	0.907	9477.090	29.642	7.158
820nM	63573.292	1.063	6623.732	21.631	10.077
200nM	55648.273	4.104	5556.449	13.405	10.148
50nM	28397.003	24.606	5125.748	27.221	5.614
13nM	9140.672	28.827	3978.997	34.130	2.354
3.2nM	6320.305	59.349	3561.350	30.253	1.808
800pM	5793.730	89.268	3316.989	29.882	1.741
200pM	5420.825	48.364	3429.219	28.846	1.602