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microwell_aspiration

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Microwell aspiration

In order to accurate aspirate small volumes it is important to make sure the air bubbles are mostly out of the lines and there are 2 pages that describe how to do this:

  1. Removing air bubbles - This technique allows you to manually perfuse the lines with a syringe.
  2. Putting together a pipette flushing macro - This uses the multichannel syringe pump to clean out the lines

https://youtu.be/KcYXge0HYjU https://youtu.be/Z4cRSgj_dKw

//aspiration_model
//valve-1_1_1_1_1_1_1_1-valvebypass
valve-1_1_1_1_1_1_1_1-bypass
//motion 96well zh 0 row 1 F3000
G1Z0F3000
G1X171.48Y176.5F5000_5
G1Z53F3000_3
//valve-1_1_1_1_1_1_1_1-valveoutput
valve-1_1_1_1_1_1_1_1-output
//syringe 10 speed 20 acc 10
sg1e10s20a10_5
//valve-1_1_1_1_1_1_1_1-valvebypass
valve-1_1_1_1_1_1_1_1-bypass
//motion waste station zh 0 row 1 F3000
G1Z0F3000
G1X235.5Y24F3000
G1Z12F3000
microwell_aspiration.1601340266.txt.gz · Last modified: 2021/08/16 15:21 (external edit)