The pipette tip imaging station is designed to measure fluorescence abundance in the pipette tip. It was designed for real-time PCR imaging but it could be used for monitoring other types of reactions as well. The fluorescence color detection scheme is FAM/FITC/FAM.

The system includes:
    1. Mechanical assembly
    2. Raspberry Pi
    3. 2 Power supplies
    4. Adjustable focus Raspberry Pi camera
    5. Camera band pass filter
    6. Filter and diffuser
    7. LED control electronics
    8. Python based MQTT client software and image processing tool for quantifying fluorescence

Example data

Here is an example of FITC antibody titration. Each of the 8 pipette tips have a different concentration of FITC. There is more info about how the system works with the LabBot here.