Fluorescence pipette tip or tube imaging
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Fluorescence pipette tip/tube imager
This imaging station is designed to measure fluorescence abundance in the pipette tip or tube attached to it. It was designed for real-time PCR imaging but it could be used for monitoring other types of reactions as well. The fluorescence color detection scheme is FAM/FITC/FAM.
The system includes:
2 Power supplies
Adjustable focus Raspberry Pi camera
Camera band pass filter
Filter and diffuser
LED control electronics
Python based MQTT client software and image processing tool for quantifying fluorescence
Here is an example of visualizing Taqman probes in a tube attached to a pipette for doing based PCR reaction detection. The model reaction is mouse beta-actin nucleic acid test using mouse cDNA. The camera can include image processing code for tracking liquid level and measuring fluorescence for FAM dyes. The animated images show the box that tracks the signal. The LabBot (3D printer) moves the tip at different Z heights as the signal is quantified.
Fluorescence pipette tip imager
Pipette tip fluorescence imager $450
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