Putting together a pipette flushing macro

From LabBot
Jump to: navigation, search

LabAutoBox Pipette Flushing Macro

Flushing the pipette tips is a good way to assess that they air bubbles are out of the lines everything is working well. Here are steps on how to build a flushing macro using the "Build Macro" page (introduced here), which will be saved as a custom macro. Generally, the steps involved are as followed:

  • 1. Move 4-way valves to bypass
  • 2. Move pipettes to waste station
  • 3. Move 4-way valves to input
  • 4. Load multichannel syringes with 500 microliters
  • 5. Turn on waste pump
  • 6. Move 4-way valves to output
  • 7. Dispense 500 microliters
  • 8. Dry pipette tips
  • 9. Turn off waste pump
Screen shot of the build macro tool

Here is a video that explains how to compile the macro.








The macro looks like this (The commented out section are those with forward slashes in front of the lines '//' ):



//valve-1_1_1_1_1_1_1_1-valvebypass
valve-1_1_1_1_1_1_1_1-bypass
//motion waste station zh 0 row 1 F3000
G1Z0F3000
G1X235.5Y24F3000
G1Z12F3000
//valve-1_1_1_1_1_1_1_1-valveinput
valve-1_1_1_1_1_1_1_1-input
//syringe 500 speed 40 acc 20
sg1e500s40a20_17
G1Z12F3000_2
//waste 2 on
wasteon
//valve-1_1_1_1_1_1_1_1-valveoutput
valve-1_1_1_1_1_1_1_1-output
//syringe 0 speed 40 acc 20
sg1e0s40a20_17
//valve-1_1_1_1_1_1_1_1-valvebypass
valve-1_1_1_1_1_1_1_1-bypass
//just dry function
touchdry Z9.5F3000ZT0T0.1
//waste 2 off
wasteoff