Inverted imaging to show inkjet spot morphology

Inverted microscopic imaging is a straightforward yet very useful tool to show how different types of spotting buffers affect spot morphology. From this point of view just using a brightfield imaging system like a microscope can clearly show the different types of polymorphs that can be shaped just by adjusting the sample liquid composition when being dispensed using a piezoelectric inkjet dispenser.

Different polymorph inkjet spot morphologies LabBot 3d printer piezoelectric dispenser

Array patterns and droplet appearance after spotting. (A) 2mg/ml mouse antibody IgG in 20mM sodium phosphate and 30mM NaCl; (B) 1mg/ml mouse antibody in PBS (137mM NaCl, 2.7mM KCl, and 11.9mM phosphate buffer); and (C) 1mg/ml bovine serum albumin 2% pluronic F68 in PBS. The top row shows the spots visualized by an inverted microscope and the bottom is the array shape on water-sensitive paper. 

 

We have been developed various tools for developing piezoelectric dispensing applications on the LabBot 3D printer platform. Some of the tools are displayed on our inkjet page:  https://labbot3dsystems.com/acoustic-dispensing-tools/.  Also we have a general purposed microfluidic and programming tool that makes it possible to run piezoelectric (inkjet) dispensers which is documented at https://labbot3dsystems.com/labautobox_microfluidics/. Demonstrated is how to attach a piezoelectric nozzle on the system. 

labbot 3d piezoelectric dispenser attaching a inkjet pipette

Diagram that shows how to attach a piezoelectric dispenser on a LabBot 3D printer, the pipette holder is modular to allow for attaching different types of pipettes

In addition to the microfluidics we also have been in working on using LabBot to do combined inverted imaging that works with the dispensers. So when the pipette is moving in the XYZ direction on the top, a camera at the bottom can move at the bottom in synchronization that can record the dispensing and visualize the polymorphs. While we use adjustable focusing cameras that work with RaspberryPi computers,  it can also be possible to move the bed up and down to control for magnification or the camera can move and down using a servo driven 3D printed linear actuator. 

LabBot 3D printer stacked corexy positioning systems this allows for inverted imaging

Inverted imaging configuration that can be set up on the LabBot, the XY moving shuttle includes a focusing camera that works with the RaspberryPi. It can include a small linear actuator for moving the camera up and down also it is possible to have the bed move up and down too.

 

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