LabBot Start Up

Good day LabBot! Time to rev you up. Here are the first steps for getting it going. 

Step 1 Turn on the pressure compensation feedback. This turns on the electrocaloric feedback loop to maintain the desired liquid level to maintain microfluidic stability

Pressure compensation vessel turning on is first step in getting the microfluidics working
The first step when turning on a LabBot (after turning on power supply) is to turn on the pressure compensation vessel feedback. So when the liquid level in this bottle is too low fills up to the desired liquid level

 

 

 

 

 

 

 

Step 2 Home the gantry to set everything to 0,0,0. This may not be necessary if you didn’t move it around when it was powered off

 

The LabBot software includes the pronterface user interface to do the homing
Click the bottom left Home to have all the axis move to the 0 positions.

 

Step 3 Move the pipette tips to the waste station

Move the pipettes to the waste position
To move the tips to the waste station, just click the button “Go to waste”

 

Step 4 Move the valves to the input position

Move the valves into the input position
Move the valves into the input position

Step 5 Make sure the multichannel syringe pump plungers are homed

Multichannel pump syringe plunger moves to zero
The software records the plunger positions so to make sure that there are no air bubbles in the syringe, it is good to home the plungers into the zero position

Step 6 Perfuse input lines with fluid to remove trapped air bubbles

 

Perfuse air out of the microfluidic input lines
Here are the steps for removing the air bubbles out of the input lines

 

Step 7 Move the valves to the bypass position

Move the valves into the bypass position microfluidics labbot 3d printer
Move the valves into the bypass position

 

 

 

 

 

 

 

Step 8 Turn on waste pump

Turn on waste button to draw excess fluid into waste
Turn on the waste button draw fluid into waste

Step 9 Move one of the valves to the close position

Move one the valves into the close position while the other valves are in the bypass position microfluidics labbot 3d printer
Move one of the valves into the close position while the other valves are in bypass

 

Step 10 Perfuse fluid into tubing connected to pipette tips to remove air bubbles

Perfuse out air bubbles from tubing connected to pipettes
To remove air bubbles in tubing connected to pipettes, draw fluid from PCV using syringe then perfuse from syringe fluid into pipettes

 

 

 

 

 

 

 

 

Step 11 Move the valve to the bypass position then work on the next valve

Move valve to bypass position
Move the valve you are working on back to the bypass position

 

Once all the pipettes are perfused then you are good to go but remember. 

Open up line between the pressure compensation vessel and the pipette tips
Once finished getting the air bubbles out., remember to position the stop cock valve to open line from PCV to pipettes and turn off the waste pump