The pressure compensation vessel (PCV) just helps control the liquid level in the pipettes. It has an electrocaloric liquid level sensor that is used as a closed feedback loop for controlling the liquid level in the bottle. So the bottle has inside a heater and heat sensor that is positioned in a waterproof container. There is an Arduino microcontroller that is used for controlling the feedback.
The system includes:
- 500ml bottle with modified hood for connecting liquid input, output, electrocaloric sensor, and air stone filter
- Bottle clamp for mounting
- Waterproof electrocaloric sensor
- Optional: peristaltic pump
- Optional: control electronics, command-line software, and power supply
This is to assure that the liquid level is stable in the pipette tips. So the idea is that the syringe pump module draws the fluid from this vessel when perfusing the pipettes. The liquid level is monitored using an electrocaloric heater that involves the probe being heated and there is a sensor that measures the temperature when the probe is out of the fluid it gets hotter that triggers the corresponding pressure compensation peristaltic pump to fill up the bottle until the temperature goes back down below the setpoint temperature.