Pressure Compensation Vessel

The pressure compensation vessel (PCV) just helps control the liquid level in the pipettes. It has an electrocaloric liquid level sensor that is used as a closed feedback loop for controlling the liquid level in the bottle. So the bottle has inside a heater and heat sensor that is positioned in a waterproof container. There is an Arduino microcontroller that is used for controlling the feedback. 

The system includes:

  1. 500ml bottle with modified hood for connecting liquid input, output, electrocaloric sensor, and air stone filter
  2. Tubing
  3. Bottle clamp for mounting
  4. Waterproof electrocaloric sensor
  5. Optional: peristaltic pump
  6. Optional: control electronics, command-line software, and power supply
pressure compensation vessel closed looped feedback to control liquid levels in pipettes for liquid handlers
Pressure compensation vessel for liquid handling which works by controlling the liquid level in the pipettes

This is to assure that the liquid level is stable in the pipette tips. So the idea is that the syringe pump module draws the fluid from this vessel when perfusing the pipettes. The liquid level is monitored using an electrocaloric heater that involves the probe being heated and there is a sensor that measures the temperature when the probe is out of the fluid it gets hotter that triggers the corresponding pressure compensation peristaltic pump to fill up the bottle until the temperature goes back down below the setpoint temperature.

pressure compensation vessel set up with LabBot 3D printer and how it can be set up with other liquid handlers
PCV set up with LabBot but it can work with 3D printers and other liquid handlers. It is part of the LabAutoBox system but availabe as a device as well. You need to adjust the level